raw 264 7 cell monoculture  (ATCC)

Journal: Materials Today Bio

Article Title: 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair

doi: 10.1016/j.mtbio.2025.102691

Figure Lengend Snippet: In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

Article Snippet: To assess the immunomodulatory properties and osteogenic capacity of each group of scaffolds evaluated in vitro , three different culture models were established: Raw 264.7 cell monoculture, rBMSCS cell monoculture and Raw 264.7-rBMSCs cell co-culture ( ). rBMSCs (RRID: CVCL_A9JU) and Raw 264.7 cells (RRID: CVCL_0493) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were authenticated according to the supplier's instructions to ensure the absence of contaminants.

Techniques: In Vitro, Co-Culture Assay, Flow Cytometry, Quantitative RT-PCR, Immunofluorescence, Fluorescence

Journal: Materials Today Bio

Article Title: 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair

doi: 10.1016/j.mtbio.2025.102691

Figure Lengend Snippet: 3 cell culture models. Raw 264.7 cell monoculture model, rBMSCS cell monoculture model, and Raw264.7-rBMSCs cell co-culture model.

Article Snippet: To assess the immunomodulatory properties and osteogenic capacity of each group of scaffolds evaluated in vitro , three different culture models were established: Raw 264.7 cell monoculture, rBMSCS cell monoculture and Raw 264.7-rBMSCs cell co-culture ( ). rBMSCs (RRID: CVCL_A9JU) and Raw 264.7 cells (RRID: CVCL_0493) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were authenticated according to the supplier's instructions to ensure the absence of contaminants.

Techniques: Cell Culture, Co-Culture Assay

Journal: Non-Coding RNA

Article Title: An Integrative Transcriptome Subtraction Strategy to Identify Human lncRNAs That Specifically Play a Role in Activation of Human Hepatic Stellate Cells

doi: 10.3390/ncrna10030034

Figure Lengend Snippet: TGFβ induced expression of HSC activation markers in different 2D or 3D culture conditions. ( a ) 3D culture of pHSC on a low attachment plate with constant shaking; ( b ) 3D culture of pHSC in high-viscosity media; ( c ) 3D culture of pHSC in Elplasia plate; ( d ) 2D culture of LX-2 cell© ( e ) 2D culture of pHSC from Donor 1; ( f ) 2D culture of pHSC from Donor 2. Cells were treated with 5 ng/mL of TGFβ for 24 h before collection for RNA isolation and RT-qPCR. For comparisons between two groups, a two-tailed, unpaired Student’s t -test was used. A p -value of more than 0.05 was considered non-significant (ns); a p -value between 0.01 and 0.05 was considered significant (*); a p -value between 0.001 and 0.01 was considered very significant (**); a p -value between 0.0001 and 0.001 was considered very extremely significant (***); a p -value of less than 0.0001 was considered extremely significant (****).

Article Snippet: For cells cultured in high-viscosity media, 3D spheroids were obtained from the Ready-to-use 3D Human Hepatic Stellate Cell Monoculture Spheroids (ScienCell Research Laboratories, Carlsbad, CA, USA), following the manufacturer’s instructions.

Techniques: Expressing, Activation Assay, Viscosity, Isolation, Quantitative RT-PCR, Two Tailed Test